5-Amino-1MQ

PEPTIDES

5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective, cell-permeable inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide and regulates cellular NAD+ homeostasis.

⚠️ For Research Use Only — This product is not intended for human consumption. By purchasing, you confirm you are a qualified researcher.

5-Amino-1MQ Research Peptide Overview

5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective, cell-permeable inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide and regulates cellular NAD+ homeostasis. As a research compound, 5-Amino-1MQ is used in preclinical studies examining NNMT’s role in lipogenesis, energy metabolism, and adipocyte differentiation pathways.

What Is 5-Amino-1MQ ?

5-Amino-1MQ was identified through medicinal chemistry efforts to develop selective NNMT inhibitors. NNMT catalyzes the methylation of nicotinamide using S-adenosyl methionine (SAM) as a methyl donor, producing 1-methylnicotinamide (1-MNA) and S-adenosyl homocysteine (SAH). By inhibiting NNMT, 5-Amino-1MQ prevents the consumption of nicotinamide and SAM, effectively increasing the intracellular pool of NAD+ precursors and preserving the SAM/SAH ratio. Published research has shown that NNMT is overexpressed in adipose tissue and plays a regulatory role in lipogenesis and adipocyte differentiation. Inhibition of NNMT with 5-Amino-1MQ has been demonstrated to alter energy flux in adipocyte models by redirecting nicotinamide toward NAD+ biosynthesis rather than methylation-dependent degradation (Neelakantan et al., Biochemical Pharmacology, 2018). Researchers use this compound to study the intersection of one-carbon metabolism, NAD+ homeostasis, and lipid biology.

5-Amino-1MQ Mechanism of Action in Research

5-Amino-1MQ competitively inhibits NNMT by occupying the nicotinamide-binding site. This prevents the methylation of nicotinamide to 1-MNA, preserving intracellular nicotinamide for NAD+ biosynthesis through the salvage pathway. Increased NAD+ availability enhances sirtuin activity (SIRT1/SIRT3), PARP-mediated DNA repair, and mitochondrial oxidative metabolism. Simultaneously, preservation of SAM levels supports global methylation reactions. In adipocyte models, NNMT inhibition has been shown to reduce lipogenic gene expression and alter differentiation programs, linking one-carbon metabolism to fat cell biology.

Key Research Applications & Findings

  • NAD+ Metabolism: Research has demonstrated that 5-Amino-1MQ increases intracellular NAD+ by redirecting nicotinamide from methylation to salvage biosynthesis pathways.
  • Lipogenesis Studies: Published studies have shown NNMT inhibition alters adipocyte differentiation and lipogenic gene expression in cell culture models.
  • Energy Metabolism: Investigators use 5-Amino-1MQ to study how NNMT activity modulates cellular energy balance through NAD+-dependent enzyme pathways.
  • One-Carbon Metabolism: The compound provides a tool for examining the interconnection between SAM-dependent methylation and nicotinamide metabolism.

5-Amino-1MQ Compared with Related Peptides

5-Amino-1MQ targets NNMT enzyme activity, operating through a mechanistically distinct pathway from SLU-PP-332 (ERR agonist for exercise-mimetic effects) and MOTS-C (mitochondrial-derived peptide affecting AMPK). Together, these compounds offer researchers complementary tools for studying different aspects of cellular energy metabolism.

Related products in the Axiom Research Supply catalog: SLU-PP-332, MOTS-C.

Research & Handling Guidelines

Dissolve in DMSO for stock solutions. Store at -20°C. Working solutions in aqueous buffers should be prepared fresh. Protect from light.

Why Choose Axiom Research Supply?

Axiom Research Supply provides 5-Amino-1MQ at ≥98% (HPLC verified), verified through independent HPLC analysis with third-party testing documentation. Every batch undergoes rigorous quality control including identity confirmation, purity assessment, and endotoxin testing. Our peptides are properly lyophilized and shipped with cold-chain protocols to maintain stability from production to your laboratory. Axiom Research Supply is committed to advancing metabolic peptide science with precision, reproducibility, and dedicated research support. Access our educational resources including the Axiom Research Supply Metabolic Peptide Research eBook for comprehensive scientific background.

Product Name 5-Amino-1MQ
Available Sizes Multiple dosage options available — see product listing
CAS Number 42464-93-1
Molecular Formula C11H13NO2
Molecular Weight 191.23 g/mol
Purity ≥98% (HPLC verified)
Physical Form Lyophilized Powder
Storage Store at -20°C, protect from light and moisture
Peptide Class NNMT Inhibitor (Small Molecule)
Key Receptor Targets Nicotinamide N-methyltransferase (NNMT) enzyme target
Research Applications NNMT enzyme inhibition, NAD+ metabolism, lipogenesis studies, cellular energy metabolism, adipocyte differentiation models
5-Amino-1MQ is used in preclinical research to study NNMT enzyme inhibition, NAD+ metabolism, lipogenesis, and adipocyte differentiation.
5-Amino-1MQ selectively inhibits nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide using SAM as a cofactor.
5-Amino-1MQ is classified as a small molecule NNMT inhibitor rather than a peptide. It is included in the Axiom Research Supply catalog as a metabolic research compound.
5-Amino-1MQ from Axiom Research Supply is for research use only and has not been approved by the FDA for any medical condition.
The CAS number for 5-Amino-1MQ is 42464-93-1.

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